Nenana basin in central Alaska is a long (90 km), narrow (12 km), and deep (7 km) sedimentary basin aligned with an active fault zone producing earthquakes. From 2015 to 2019, 13 broadband seismic stations were deployed in the region as part of the Fault Locations and Alaska Tectonics from Seismicity project. These stations recorded a wide range of earthquakes, including 3–4 directly below the basin as well as several regional earthquakes . These 43 local and regional earthquakes, in addition to five teleseismic events and continuously recorded ambient noise, provide a data set that we use to quantify the response of Nenana basin to the seismic wavefield. We calculate spectral ratios between each station and a bedrock reference station for 48 earthquakes. We find amplification of 11–14 dB (amplification ratio 3.5–5.0) for low frequencies (0.1–0.5 Hz), and 8–15 dB (amplification ratio 2.5–5.6) for high frequencies (0.5–4.0 Hz) on the vertical component. At low frequencies, amplification of the earthquake wavefield agrees well with amplification of seismic noise, with both data sets exhibiting stronger amplification on the horizontal components, in comparison with the vertical component. Furthermore, stations overlying the deeper part of the basin exhibit stronger amplification, whereas stations at the margin of the basin exhibit minimal low‐frequency amplification. At higher frequencies, amplification occurs at both deeper basin stations and also marginal basin stations. Our study establishes a catalog of diverse events for future theoretical and numerical studies that can use Nenana basin to better understand the complex influence of sedimentary basins on the seismic wavefield.