The delta 13 C-delta 18 O- 87 Sr/ 86 Sr variations around carbonate-hosted deposits can in theory help characterize ore-stage gangue minerals and discriminate between single fluid or two-fluid mixing models during mineralization. This paper reports on the combined use of delta 13 C-delta 18 O- 87 Sr/ 86 Sr data in dolostone within the Gays River deposit and up to 30 km around it. The deposit, at the southern margin of the Maritimes basin, is hosted in carbonate banks of the Gays River Formation, a unit of the Visean Lower Windsor Group in Nova Scotia. A previous delta 13 C-delta 18 O study restricted to the Gays River deposit (and independant from an 87 Sr/ 86 Sr investigation) had previously led to the conclusion that a single brine was responsible for mineralization.The present isotopic investigation was governed by the paragenetic succession which includes presulfide dolomite, synsulfide calcites--both uniform and zoned under cathodoluminescence, and postsulfide calcite uniform under cathodoluminescence. Results for presulfide dolomite (avg delta 13 C = +3ppm, delta 18 O (sub V-PDB) = -6ppm, and 87 Sr/ 86 Sr = 0.7080) plot near the normal marine isotopic ratios, whereas postsulfide calcite shows slightly more radiogenic 87 Sr/ 86 Sr ratios (avg = 0.7090). At the deposit isotopic results for calcite associated with the high-grade sulfide zone cluster around a delta 18 O (sub V-PDB) of -- 17.0 per mil and an 87 Sr/ 86 Sr of 0.7120, and clearly characterize the mineralizing system. Regional C, O, and Sr isotope data are similar to those at the deposit and indicate therefore a regional flushing of the Gays River Formation by fluids of the mineralizing system.The overall isotopic variation through time from pre- to syn- then postsulfide conditions in the delta 18 O -- 87 Sr/ 86 Sr, delta 13 C-delta 18 O and delta 13 C- 87 Sr/ 86 Sr diagrams is interpreted to be similar for pre- and postsulfide conditions, marked by a warm, slightly radiogenic parent brine. This chemical system was disturbed by a much more radiogenic and high-temperature fluid during mineralization. Although most of the mineralizing trend can be explained either by a single-brine model or a mixing brine model, the mixing model allows for a much simpler evolution if the suite of conditions--on a regional basis--is considered through time."Show me your delta 18 O- 87 Sr/ 86 Sr profile, and I will tell you what you drink!" (adapted old spanish proverb).