—The paper is concerned with a geochemical study of apoultrabasic metasomatites of the Ospa–Kitoi, Parama, and Ust’-Kelyana ophiolite massifs located in the southern folded framing of the Siberian craton. The isotope (O, C, H, Sr, and Rb) systems of dunites, serpentinites, nephrites, listvenites, and talc–carbonate rocks are studied. The isotopic composition of oxygen in olivines from dunites is characterized by δ18O = 4.6–5.5‰. The δ18O values of serpentinites (4.67–7.35‰) point to the mantle genesis of fluids and might have been inherited from ultrabasic rocks. Nephrites are slightly enriched in heavy oxygen isotope (δ18O = 6.13–9.54‰). This indicates that their fluid phase was transported from serpentinites and captured a small portion of the crustal component. The widest variations in δ18O values, from 8.12 to 17.46‰, are observed in minerals from listvenites. Carbonates from these rocks show a highly heterogeneous isotopic composition of oxygen (δ18O = 12.9–18.8‰) and carbon δ13C = –2.8 to +2.8‰). These rocks formed with the contribution of metamorphogenic fluids. According to the isotopic composition of hydrogen, the examined serpentinites are divided into two groups: with δD values specific to “magmatic water” (δD = –73.50 to –85.00‰) and those typical of meteoric fluids (δD = –151.90 to –167.20‰). The listvenites are characterized by low Rb and high Sr contents. Their 87Sr/86Sr values (0.70702–0.70971) indicate the contribution of a crustal source. The study of fluid inclusions in minerals from listvenites has shown that the rocks formed under relatively low-temperature conditions. The homogenization temperatures of fluid inclusions in quartz and magnesite from listvenites of the Ospa–Kitoi massif are 184–290 ºC and 122–182 ºC, respectively. In the Parama massif, the homogenization temperature of fluid inclusions in quartz is 130–170 ºC. The solutions that formed listvenites of the Ospa–Kitoi massif were slightly saline (TDS = 2.9–8.4 wt.% NaCl eq.), with NaCl and Na2CO3 being the main salt components.