Analysis of geological and geophysical data on the boundary zones between the Eurasian plate and other plates shows poor knowledge of the deep structure of the region. This information will help to refine the position of the plate boundaries and the structure of the Earth’s crust and mantle. We present data on the seismicity and deep structure along the deep seismic sounding (DSS) profile running across the boundary between the Eurasian and Okhotsk plates. A comprehensive analysis of the DSS materials and seismic data shows a significant expression of this boundary zone both in the deep Earth’s crust structures and in the Moho. A zone of anomalous seismicity and deep structure extends along the DSS profile for several hundred kilometers. We have refined the position of the main boundary between the Eurasian and Okhotsk plates, which passes approximately along 144° E.