The ecosystem services offered by honey-producing bees are highly significant for the fertilization of flowers to produce fruits and seeds. The pollen found on bees and in honeycombs and honey represents the type of vegetation visited by the bees. The pollen diversity of honeycombs built by four honey-producing bees of the west coast and Western Ghats of India was evaluated to identify the floral sources. A spectrum of 54 pollen types (belonging to 52 genera in 34 families) was identified on the west coast, while 94 pollen types (belonging to 73 genera in 41 families) were identified in the Western Ghats. The results show that the bees of the four species on the west coast visited a limited number of plant species to gather nectar but included a higher quantity of total pollen of specific plant species. In the Western Ghats, the same species of bees visited more plant species but obtained lower quantities of pollen of specific plant species. This study shows that the honey-producing bees of the west coast and the Western Ghats prefer multifloral nectar for the production of honey. Floral resources for honey-producing bees are more diverse in the Western Ghats than on the west coast of India. Such differences might influence the quality (physical and nutraceutical) of honey produced by the bees in different geographic regions.