The pollen morphology of Podostemum (Podostemaceae) was analysed with the aim of providing detailed descriptions of pollen for all species of the genus. Pollen grains were obtained from anthers of flower buds, acetolysed, and examined using light and scanning electron microscopy. The pollen grains are dyads or monads, small or medium sized, with shape ranging from 14.7 to 32.3, prolate spheroidal, oblate spheroidal, prolate, subprolate, with a circular, subtriangular or triangular amb. Pollen grains are tricolpate, and the colpus membrane conspicuously ornate. The exine is thin, spiculate with mamilloid elements with acute apices. Hierarchical cluster analysis (HCA) was used to divide the main pollen taxa into three groups based on pollen morphology. Statistically distinctive taxa were identified using principal components analysis (PCA). Pollen analysis revealed a significant overall correspondence between pollen variation amongst species of Podostemum and phylogenetic patterns.

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