To further our knowledge of ore genesis in one of Australia's preeminent ore districts, we have completed a comprehensive geochemical study of ore-related porphyritic intrusions from the Archaean Kanowna Belle and Sunrise Dam gold deposits (both >10 Moz), Eastern Goldfields, Western Australia. Zircon samples (including samples from the newly developed Velvet mine) with ages ranging from 2.8 to 2.2 Ga, were investigated for O-OH isotopic signatures, trace element abundance, and U-Th-Pb compositions to elucidate the nature of the magmatic source and ore-related fluid. These intrusions have similarly high Sr/Y and La/Yb ratios to adakites from the Aleutian and Cook Islands, but lower Mg# values and higher K2O contents, suggesting they were derived from partial melts in a thickened crust. The modern analogs are post-collisional, high-Sr/Y granitoid porphyries in southern Tibet. Magmatic zircons have intermediate δ18O values (+5‰ to +6.3‰), and estimated magmatic crystallization temperatures (Ti-in-zircon) in between 660–760 °C. They are interpreted as having crystallized from positive δ18O magmas during water-fluxed melting of juvenile lower crust. Hydrothermal fluid modified zircons are texturally indistinguishable from magmatic zircons, but their trace element, OH, and isotopic compositions are distinct. The involvement of hydrothermal fluid in zircon growth is evidenced by a negative correlation between OH content and δ18O. In addition, the studied hydrothermal fluid modified zircons are characterized by high La contents, flat rare earth element patterns, weak Ce anomalies, and high Eu/Eu* ratios, suggesting they were related to a high-temperature, Zr-saturated, high-Eu, Cl-rich, and low-pH hydrothermal fluid. Such fluids are common in eastern Yilgarn gold camps.