Two new isocrinids are described from the Lower Cretaceous Agrio Formation of the Neuquén Basin, west-central Argentina. Isocrinus (Chladocrinus) covuncoensis new species is based on several beautifully preserved specimens from Valanginian beds of the Pilmatué Member. It is characterized by a small size, multiramose crown with six arm divisions, 240 arm tips, mostly isotomous branching, seven (or rarely eight) secundibrachials, smooth and stout column, short noditaxis, and pentalobate columnals. The species occurs in a 30 m thick interval of cross-bedded sandstones and mixed clastic-carbonate sediments that represent the migration of large, tidally influenced, subaqueous dunes developed in the offshore. Sudden burial of crinoids that dwelled on the dune toes and interdunes, possibly by the acceleration of the lee face migration, provided the exceptional preservation of specimens and thus this finding can be considered as a local crinoid Konservat Lagerstätte. Isocrinus (Chladocrinus) pehuenchensis new species is described from a single articulated specimen preserved in a silty calcareous concretion collected from a late Hauterivian concretion level of the Agua de la Mula Member. It is characterized by isotomous branching, eight or nine secundibrachials (IIBr), slender column ornamented with medial ridge of fine tubercles, interradius acuminated with fine tubercles on its tip, short noditaxis, and pentastellate columnals. It is associated with low-energy fall-out deposits in the offshore. The excellent state of preservation was due to an early cementation process by carbonate that enhanced lithification around the specimen.