This paper describes 19 brachiopod species (including six indeterminate species) in 15 genera and one indeterminate genus from the basal Itaituba Formation at the Caima Quarry 1 section of Itaituba, Amazon Basin, Brazil. The faunal correlations of the brachiopods and the associated fusulinids and conodonts indicate a late Chesterian (late Serpukhovian) age for the described fauna, therefore confirming for the first time the presence of uppermost Mississippian rocks in the Amazon Basin. A new species, Composita caimaensis, is created, and two species, Inflatia cf. gracilis and Marginovatia cf. catinulus, are described for the first time from the Amazon Basin. The Amazon brachiopods appear to be of strong affinity with coeval faunas of the North American midcontinent.