Moderately well-preserved Middle Triassic radiolarians were recovered from bedded limestone exposed at about 3 km west of Kefamenanu, West Timor, Indonesia. This limestone probably from the Aitutu Formation is considered to be an allochthonous block and is embedded in the Neogene Bobonaro Complex. The radiolarian fauna in this limestone is characterized by abundant radiolarians of typical Tethyan forms and is identical to that of the early Fassanian (early Ladinian) of European Tethys and other related faunas reported from the Philippines, Russian Far East, and Japan. The Aitutu Formation is thought to be deposited in an ocean environment dominated by a warm-water current system originating from the low latitude Tethyan realm. Fifty-nine species belonging to 34 genera, including five unidentified genera, are systematically treated, among them, five new species; Parentactinia suparkai, Pseudostylosphaera timorensis, Cryptostephanidium? megaspinosum, Tetrarchiplagia compacta, and Planospinocyrtis kefaensis.