Pupae of the nonbiting midge Chaoborus are reported from the middle Eocene (Claibornian) Tallahatta Formation in Benton County, Mississippi. These pupae are placed within the genus Chaoborus because the shape of the respiratory organs, length of the abdomen, and shape of the anal paddles closely resemble other species of this extant genus. This occurrence represents the oldest record of Chaoborus pupae and the first record of fossil Chaoborus from North America. The flora and fauna found associated with the fossil pupae along with the known habitats of extant Chaoborus pupae indicate a lentic environment. This interpretation corresponds to the depositional environment of similar Eocene-aged clay deposits in western Tennessee.