Travertine depositional systems are the surficial evidence of an active geothermal system developing in the upper crust. They are often associated with further geothermal manifestations, such as gas vents (mainly CO2) and thermal springs from which the travertine develops. The distribution of the geothermal manifestations and the growth of the travertine deposits are strictly controlled by active, potentially seismogenic structures. Thus, the study of the geothermal manifestations including travertine deposits can provide information on the seismotectonic setting of an area, revealing the fault occurrence, orientation, and kinematics. In this paper we present a study focused on the relationships among Late Pleistocene-Holocene travertine deposits, still in deposition, thermal springs, gas vents and the Neogene-Quaternary faults in the Monte Amiata Volcano-Geothermal area. Results from a new detailed field mapping aimed at (i) reconstructing the isolated travertine bodies in an area of about 2 km2, in terms of geometry, age (by U/Th radiometric dating) and depositional setting, and (ii) refining the structural dataset on the substratum rocks (formed by Tuscan and Ligurian Units) allowed to define the main faults controlling the geothermal manifestations and related travertine deposition. We highlight fault arrays compatible with a releasing step-over zone which geometry has been partly inherited by previous structures. At the same time, the study on travertine deposits allowed to recognize Late Pleistocene-Holocene fissure ridge-type and mound-type travertine deposits developed along the traces of faults, which still control, locally, gas emission and the location of thermal springs. We also discuss the tectonic setting in the framework of the evolution of the Monte Amiata Volcano-Geothermal area and in the seismotectonic setting.