Because shale gas content plays a very important role in the evaluation of gas shale potential, its calculation and prediction become obligatory. We used two predictive models, namely, the Langmuir and Ambrose models, to calculate the shale gas content. The parameters involved in these two models are calculated by various experiments and analytic methods, including indirect prediction, the isothermal adsorption test, X-ray diffraction analysis, total organic carbon (TOC) measurement, pyrolysis, and porosity measurement. Then, a new calculation model that is applicable to shales in the Kuqa Depression, Tarim Basin, is established. Further research on influential factors of gas content in well YN2 is implemented. The result indicates that the gas content of terrestrial shales is more influenced by TOC abundance than by the content of clay minerals and quartz. The main parameters in the new calculation model are the TOC, depth, porosity, and gas saturation. The Jurassic shale gas in well YN2 is speculated to be mainly adsorption gas, with a dominant proportion of 75%–90% in the total gas content. As the formation depth increases, the free-gas content rises continuously, whereas the adsorption gas content first increases and then approaches the equilibrium value or even tends to decrease slightly. Based on the foregoing results, the target layer, the Yengisar Formation, is predicted to possess an enormous amount of shale gas potential, with an average total gas content of .