The Shiranish Formation consists of mudstones and wackestones in the central Euphrates Graben which are rich in organic carbon. Here the Shiranish Formation is more than 700 m thick with a minor increase in organic maturity with depth. The Shiranish Formation sediments are characterized by a continuously increasing hydrogen index to the top whereas the oxygen index is markedly lower in the Upper Shiranish Formation (USF). The Lower Shiranish Formation (LSF) is characterized by lower hydrogen indices and higher oxygen indices relative to the USF. These organic geochemical characteristics enable a rough subdivision into a lower and an upper part of the Shiranish Formation. Furthermore, mineralogical results enable a subdivision of the USF into two parts (USF-1, lower part; USF-2, upper part) each with individual mineralogical signatures due to a modified depositional environment and differing diagenetic history. The LSF resembles mineralogically the USF-2. Ankerite, together with higher pyrite contents in the LSF and USF-2, reflect similar diagenetic pathways which were controlled by higher clay contents. During early diagenesis, a traceable conversion of metabolizable organic matter led to mineral assemblages due to significant methanogenesis. Intervals in the USF with total organic carbon (TOC) contents up to around 4% and hydrogen indexes up to 500 mg HC/g TOC indicate the presence of very good potential source rock intervals for oil generation. Additionally, intervals of the LSF also contain gas-prone organic material. Bulk kinetic investigations show a broad activation energy of the LSF and a narrow activation energy pattern for the USF for hydrocarbon generation. Furthermore, the predicted petroleum formation temperatures are 136°C for the USF and 144°C for the LSF, respectively. This corresponds to c. 630 m difference in burial depth for petroleum formation. These differences in activation energies and corresponding depth to reach oil window maturity are controlled by facies, and less by maturity.