The upper Cambrian Maynardville limestone was investigated through a strike length of 8½ miles in Jefferson and Sevier counties, Tennessee. A detailed composite columnar section shows five zones in the formation, from the base upward: (1) interbedded limestone and shale, (2) massive Stromatolitic limestone, (3) oölitic and conglomeratic limestone, with thin Stromatolitic beds, (4) massive and thick-bedded limestone, with a few dolomitic and Stromatolitic layers, and (5) thin- to medium-bedded dolomite, with very little limestone. Extensive Stromatolitic bioherms in Zone 2 contain subcircular forms, presumably the fossil outlines of algal colonies. Many intercolony fillings are composed of calcareous oölites and clastic sediments in a fine-grained calcitic matrix; others consist of dolomite and calcareous shale. Similar Stromatolitic limestones were found in eight other outcrop belts of the Maynardville in East Tennessee. Five localities are listed where the algal colonies are well exposed in the outlying belts.