Continental magmatic arcs are characterized by the accretion of voluminous mantle-derived magmatic rocks and the growth of juvenile crust. However, significant volumes of meta-sedimentary rocks occur in the middle and lower arc crust, and the contributions of these rocks to the evolution of arc crust remain unclear. In this paper, we conduct a systematic study of petrology, geochronology, and geochemistry of migmatitic paragneisses from the eastern Gangdese magmatic arc, southern Tibet. The results show that the paragneisses were derived from late Carboniferous greywacke, and underwent an early Cenozoic (69–41 Ma) upper amphibolite-facies metamorphism and partial melting at pressure-temperature conditions of ~11 kbar and ~740 °C, and generated granitic melts with enriched Hf isotopic compositions (anatectic zircon εHf(t) = −10.57 to +0.78). Combined with the existing results, we conclude that the widely distributed meta-sedimentary rocks in the eastern Gangdese arc deep crust have the same protolith ages of late Carboniferous, and record northwestward-decreasing metamorphic conditions. We consider that the deeply buried sedimentary rocks resulted in the compositional change of juvenile lower crust from mafic to felsic and the formation of syn-collisional S-type granitoids. The mixing of melts derived from mantle, juvenile lower crust, and ancient crustal materials resulted in the isotopic enrichment of the syn-collisional arc-type magmatic rocks of the Gangdese arc. We suggest that crustal shortening and underthrusting, and the accretion of mantle-derived magma during the Indo-Asian collision transported the supracrustal rocks to the deep crust of the Gangdese arc.