Pore fluids extracted from a 456 m sediment core, recovered within the framework of a multinational and International Continental Scientific Drilling Program (ICDP) co-sponsored effort at the bottom of the terminal Dead Sea, recorded the chemical variations in the deep lake over the past 220 k.y. Mg2+ and Br– were shown to be conservative in the pore fluids, increasing in concentration during interglacial periods, diluting during glacials, and providing excellent proxies for deep lake net water balance changes. Furthermore, the Na/Cl ratio recorded the process of halite precipitation and dissolution induced by these hydrological changes. Mg2+ and Br– records follow a glacial-interglacial pattern, such as observed in atmospheric CO2 concentrations and global sea-surface temperatures, albeit with a phase offset. At the end of the last interglacial (ca. 116 ka), there is a delay in onset of dilution of the deep lake, most likely due to the limnological transition from holomictic to meromictic conditions. The increase in deep lake concentrations at Last Glacial Termination I is delayed as a result of freshwater input into the deep lake during the cooler Younger Dryas period. There is a persistent relationship between precipitation in the watershed and North Atlantic sea-surface temperatures, similar to conditions observed over the past instrumental record. Deviations from the long-term trends occurred during interglacial periods, Marine Isotope Stages MIS 5e and MIS 1, when the deep Dead Sea was significantly diluted, and coincided with Mediterranean sapropel layers S5 and S1.