We have discovered a robust microcrystalline record of the early genesis of North American lithosphere preserved in the U-Pb age and oxygen isotope zoning of zircons from a lower crustal paragneiss in the Neoarchean Superior province. Detrital igneous zircon cores with δ18O values of 5.1‰–7.1‰ record creation of primitive to increasingly evolved crust from 2.85 ± 0.02 Ga to 2.67 ± 0.02 Ga. Sharp chemical unconformity between cores and higher δ18O (8.4‰–10.4‰) metamorphic overgrowths as old as 2.66 ± 0.01 Ga dictates a rapid sequence of arc unroofing, burial of detrital zircons in hydrosphere-altered sediment, and transport to lower crust late in upper plate assembly. The period to 2.58 ± 0.01 Ga included ∼80 m.y. of high-temperature (∼700–650 °C), nearly continuous overgrowth events reflecting stages in maturation of the subjacent mantle root. Huronian continental rifting is recorded by the youngest zircon tip growth at 2512 ± 8 Ma (∼ 600 °C) signaling magma intraplating and the onset of rigid plate behavior. This >150 m.y. microscopic isotope record in single crystals demonstrates the sluggish volume diffusion of U, Pb, and O in zircon throughout protracted regional metamorphism, and the consequent advances now possible in reconstructing planetary dynamics with zircon zoning.