We investigated, by means of optical dating, the chronostratigraphic nature of the sequence boundary associated with the last glacial in a sandy to gravelly compound paleovalley fill, just landward of the highstand shoreline in the Rhine-Meuse Delta (Netherlands). Laterally extensive fluvial strata deposited during oxygen isotope stage 4, coeval with a major sea-level fall, unconformably overlie estuarine deposits from stage 5 or fluvial deposits from the penultimate glacial (stage 6). These chronostratigraphic relationships differ substantially from widely used models and indicate (1) that sequence-boundary formation in this setting was associated with the onset of pronounced sea-level fall, shortly after 80 ka; (2) that the time gap represented by the sequence boundary may be extremely small (<10 k.y.); (3) that the age of the sequence boundary may decrease both updip and downdip of the highstand shoreline; and (4) that our study does not provide viable diagnostic criteria for a sea-level–controlled sequence boundary above the falling-stage systems tract. Despite the high-frequency, high-amplitude glacio-eustatic regime that might be considered ideal for the formation of an unambiguous unconformity, the last sequence boundary in this setting is commonly cryptic.