Geochemical mapping has revealed the presence of two distinct units within the mantle section of the Troodos massif, Cyprus. The first unit (U1) is composed of spinel lherzolite (Cr# [Cr/(Cr+Al)] of chrome spinels = 0.22–0.28), which contains numerous dunite bodies accompanied by zones of clinopyroxene-bearing harzburgite (U1-harzburgite). The second unit (U2) mainly consists of refractory clinopyroxene-poor harzburgite (U2-harzburgite; Cr# of chrome spinels = 0.51–0.70). The U1 spinel lherzolite is interpreted as residue after mid-ocean ridge basalt extraction, which was tectonically juxtaposed with U2. It was not involved in production of suprasubduction-type Troodos magmas, but has subsequently been modified by their percolation. The dunite bodies and related zones of U1-harzburgite might represent pathways of these melts in the mantle. The U2-harzburgite could have resulted from reaction between host rock and melts.