At some time during the Pleistocene Epoch, a part of the modern Ohio River drainage system in Ohio and West Virginia developed in response to impoundment of the ancestral Teays River drainage system. Rhythmites formed in the lacustrine slackwaters and remain today, extending as much as 150 to 200 km upstream from the Pleistocene ice front, in Teays Valley, West Virginia. A total of 303 oriented paleomagnetic specimens represent a composite stratigraphic section from the Minford Silt Member of the Teays Formation in Teays Valley. Of these, 224 specimens carry a stable reversed magnetization due to detrital magnetite and hematite. Two distinctive lithologic intervals with definitive magnetic intensities were found in the stratigraphic section; thick, light-colored rhythmites carry six times more remanent magnetization intensity than thin, dark-colored rhythmites, reflecting variations in the ratio of magnetite to hematite. From the Pleistocene paleomagnetic chronology, the glacial diversion of the Teays River in Ohio and West Virginia took place during the Matuyama reversed polarity chron, in the time interval between 0.79 and 1.60 Ma, the change attributed to an Early Pleistocene age, most probably the F or G glaciation. We propose that the Minford Silt was deposited between 0.79 and 0.88 Ma.