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We present the results of integrated biostratigraphic (planktonic foraminifera, calcareous nannofossils, and dinoflagellates), magnetostratigraphic, and cyclostratigraphic analyses of the lower part of Monte Cagnero section (Umbria-Marche Apennines of Italy), a continuous and complete succession of pelagic limestone and marls that provides the means for an accurate and precise astrochronologic calibration of the Eocene-Oligocene transition. This 38.5-m-thick section overlaps the Oligocene section, which, at meter level 188, contains the Rupelian-Chattian boundary corresponding to the O4-O5 planktonic foraminiferal zonal boundary within the upper half of magnetochron C10n. The Eocene-Oligocene boundary at Monte Cagnero, as defined by the last occurrence of hantkeninid planktonic...

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