ABSTRACT At present, the Global Stratotype Section and Point (GSSP) for the base of the Bartonian remains the only GSSP of the Paleogene System to be defined by the International Subcommission on Paleogene Stratigraphy (ISPS) and the International Commission on Stratigraphy (ICS). Here, we present the results of an integrated, high-resolution study of calcareous plankton and benthic foraminifera biostratigraphy and a detailed magneto-, chemo-, and cyclostratigraphic analyses carried out through the upper Lutetian to the upper Priabonian pelagic sediments of the Bottaccione Gorge section near Gubbio, central Italy, to check its stratigraphic completeness and constrain in time the optimal interval for defining and positioning the GSSP for the base of the Bartonian Stage. The high-resolution and solid integrated stratigraphic framework established at Bottaccione confirmed the completeness of the section, which meets the ICS recommendations for a potential designation as a GSSP for the base of the Bartonian Stage. Thus, the Bottaccione section was compared with the parastratotype section of the Bartonian in its type area, Alum Bay, UK. On this basis, two reliable criteria for defining and positioning the Bartonian GSSP at Bottaccione are provided: (1) the base of magnetic polarity chronozone C18r as the primary correlation criterion and (2) the base of the calcareous nannofossil Dictyococcites bisectus , which defines the CNE14/CNE15 zonal boundary as a secondary correlation criterion.