ABSTRACT Lignin phenol, pollen, and diatom analyses were performed on dated sediments (13,533–8993 cal yr B.P.) recovered from Fallen Leaf Lake, California. This multiproxy data set constrains the end of the Tioga glaciation in the Lake Tahoe Basin and reconstructs the response of the region’s aquatic and terrestrial ecosystems to climatic changes that accompanied the Younger Dryas, the end of the Pleistocene, and early Holocene warming. From the Pleistocene to the Holocene, lignin concentrations and syringyl/vanillyl (S/V) ratios increased, while cinnamyl/vanillyl (C/V) ratios and the lignin phenol vegetation index (LPVI) decreased, recording the proliferation of woody plant material and, particularly, the expansion of angiosperms as the Tioga glaciation ended and temperatures warmed. This interpretation is constrained by lignin phenol analyses of plant material from Fallen Leaf Lake’s present-day watershed. Complementary palynological analyses show a transition from a gymnosperm-dominated landscape to a more mixed angiosperm-gymnosperm vegetation assemblage that formed as closed canopy forests became more open and grasses and aster colonized meadows. Aquatic flora assemblages, in the form of greater amounts of green algae and greater percentages of diatom phytoplankton, indicate increased levels of lake primary productivity in response to warming. Principal component analysis (PCA) distinctly resolves the Pleistocene from the Holocene diatom flora. The Pleistocene flora is dominated by cyclotelloids and low-mantled Aulacoseira species that are rare in Fallen Leaf Lake today, but common at higher and colder elevations that may resemble the Pleistocene Fallen Leaf Lake. The Holocene diatom flora is dominated by Aulacoseira subarctica .

Abstract: Organic compounds are ubiquitous in the Earth’s surface, sediments and many rocks, and preserve records of geological, geochemical and biological history; they are also critical natural resources and major environmental pollutants. The naturally occurring stable isotopes of volatile elements (D, 13 C, 15 N, 17,18 O, 33,34,36 S) provide one way of studying the origin, evolution and migration of geological organic compounds. The study of bulk stable isotope compositions (i.e. averaged across all possible molecular isotopic forms) is well established and widely practised, but frequently results in non-unique interpretations. Increasingly, researchers are reading the organic isotopic record with greater depth and specificity by characterizing stable isotope ‘structures’ – the proportions of site-specific and multiply substituted isotopologues that contribute to the total rare-isotope inventory of each compound. Most of the technologies for measuring stable isotope structures of organic molecules have been only recently developed and to date have been applied only in an exploratory way. Nevertheless, recent advances have demonstrated that molecular isotopic structures provide distinctive records of biosynthetic origins, conditions and mechanisms of chemical transformation during burial, and forensic fingerprints of exceptional specificity. This paper provides a review of this young field, which is organized to follow the evolution of molecular isotopic structure from biosynthesis, through diagenesis, catagenesis and metamorphism.