Abstract Penguins are by far the most dominant group of marine vertebrates in the Eocene La Meseta Formation (Seymour Island, Antarctica). We analysed the penguin fauna recovered there from both a systematic and a biostratigraphic point of view. We have added two new species ( Tonniornis mesetaensis and T. minimum ) and have defined a biostratigraphic unit, the Anthropornis nordenskjoeldi Biozone. This interval of strata, easily distinguishable by the numerous occurrence of penguin bones and the phosphatic brachiopod Lingula , is located nearly 30–35 m below the top of the 145 m-thick Submeseta Allomember. The highest morphological and taxonomic penguin diversity living sympatrically (organisms that live simultaneously in the same place), including giant and tiny species, is documented in this interval. Fossil penguins bones studied in this paper, recovered from rocks interpreted as shallow-marine deposits, accumulated between 34.2 and 36.13 Ma (late Late Eocene).

Abstract Eocene Antarctic penguins, at least 10 species in six genera, are known only from the La Meseta Formation, Seymour Island, Antarctic Peninsula. They are most numerous (in terms of individuals, body sizes and taxa) in Late Eocene strata. Specimens from three species and phylogenetic analysis presented in this work shed new light on the systematics and evolution of Antarctic Sphenisciformes. The earliest reported bones of giant penguins from the genus Anthropornis set the conservative estimate of its divergence time at c . 53 Ma (Early Eocene). They also document the oldest known appearance of quite a high diversity of Sphenisciformes; altogether, three morphotypes (differing in size) have been found within the same sampling locality. A newly described, relatively small and intriguingly elongated, tarsometatarsus from the Late Eocene of the La Meseta Formation, belonging to another genus of large-sized Antarctic penguins ( Palaeeudyptes ), suggests the possible existence of an unnamed species within this long-established genus. The phylogenetic analysis based on tarsometatarsal features shows that the relationship between ‘ Archaeospheniscus ’ wimani and three species of Delphinornis (all of them co-existed during the Late Eocene time period) does not appear to be close enough to justify merging them into a single genus (as was recently postulated). Supplementary material: An annotated data matrix used for the phylogenetic analysis is available at www.geolsoc.org.uk/SUP18599