Abstract Microbially induced sedimentary structures (MISS) are primarily known from transitional marine (tidal) and shallow-marine settings. New results from the ca. 1.1 Ga Midcontinent Rift System of North America extend their record into wholly terrestrial depositional settings, including capping paleosols, paludal environments, and alluvial sedimentary units. The MISS are present on both sides of present-day Lake Superior, at Good Harbor Bay, Minnesota, and at Copper Harbor, Michigan, indicating a regionally widespread biosphere. MISS in the Midcontinent Rift System include abraded Kinneyia, pustulose mound structures, multidirectional wave ripples, textured bedding plane surfaces, and stromatolites. Organic carbon is also preserved in MISS from both sides of the rift, and in finely laminated sedimentary strata, indicating that the biosphere extended also into the hinterland. Independent climatic evidence indicates a temperate setting; thus, the new MISS extend both the depositional and environmental niches where MISS are preserved.