Abstract Oligocene and Lower Miocene deposits in the Paratethys are important source rocks, but reveal major stratigraphic and regional differences. As a consequence of the first Paratethys isolation, source rocks with very good oil potential accumulated during Early Oligocene time in the Central Paratethys. Coeval source rocks in the Eastern Paratethys are characterized by a lower source potential. With the exception of the Carpathian Basin and the eastern Kura Basin, the source potential of Upper Oligocene and Lower Miocene units is low. In general, this is also valid for rocks formed during the second (Kozakhurian) isolation of the Eastern Paratethys. However, upwelling along a shelf-break canyon caused deposition of prolific diatomaceous source rocks in the western Black Sea. Overall, Oligocene–Lower Miocene sediments in the Carpathian Basin (Menilite Formation) can generate up to 10 t HC m −2 . Its high petroleum potential is a consequence of the interplay of very high productivity of siliceous organisms and excellent preservation in a deep silled basin. In contrast, the petroleum potential of Oligocene–Lower Miocene (Maikopian) sediments in the Eastern Paratethys is surprisingly low (often <2 t HC m −2 ). It is, therefore, questionable whether these sediments are the only source rocks in the Eastern Paratethys.

Abstract: Compound-specific isotope analysis (CSIA) of individual organic compounds is a powerful but underutilized tool in petroleum exploration. When integrated with other organic geochemical methodologies it can provide evidence of fluid histories including source, maturity, charge history and reservoir processes that can support field development planning and exploration efforts. The purpose of this chapter is to provide a review of the methodologies used for generating carbon and hydrogen isotope data for mid- and high-molecular-weight n -alkanes. We discuss the factors that control stable carbon and hydrogen isotope compositions of n -alkanes and related compounds in sedimentary and petroleum systems and review current and future applications of this methodology for petroleum exploration. We discuss basin-specific case studies that demonstrate the usefulness of CSIA either when addressing particular aspects of petroleum exploration (e.g. charge evaluation, source rock–oil correlation, and investigation of maturity and in-reservoir processes) or when this technique is used to corroborate interpretations from integrated petroleum systems analysis, providing unique insights which may not be revealed when using other methods. CSIA of n -alkanes and related n -alkyl structures can provide independent data to strengthen petroleum systems concepts from generation and expulsion of fluids from source rock, to charge history, connectivity, and in-reservoir processes.