Abstract The main Karoo Basin of South Africa and Lesotho preserves c. 120 myr of Earth's history. The sedimentary rocks of its Karoo Supergroup record massive environmental changes from the glacial Carboniferous to desert dunes and fiery flood basalts in the Early Jurassic. From the early Permian, the Karoo Basin was gradually filled with fluvial and lacustrine deposits, and the alluvial plains were successively colonized by a diverse suite of plants and animals. The fossils of these ancient inhabitants and their behavioural traces form an astounding Gondwanan geoheritage legacy in southern Africa, providing fossil evidence for the moving lithospheric plates and the effects of four mass extinctions and their subsequent biotic recovery. Here, we present six Karoo sites of global geoscientific importance that best display that heritage, with the caveat that these sites only touch upon the Karoo riches that are available for academic research and the emerging palaeotourism industry. It is our hope that these sites will become anchor points for a sustainable geoheritage future in southern Africa.