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Efficacy of sonication-assisted sieving on Quaternary pollen samples

Angelina G. Perrotti, Taylor Siskind, Mary Katherine Bryant and Vaughn M. Bryant
Efficacy of sonication-assisted sieving on Quaternary pollen samples
Palynology (October 2018) 42 (4): 466-474

Abstract

Debris in archaeological pollen samples can obscure pollen grains during microscopic analysis. When attempting to remove debris smaller than 10 microns from a pollen sample, screen openings can be too small, requiring the sample to be agitated in order to facilitate its passing through the screen. We recently developed a new method that uses a Branson (super C) S450 sonicating disruptor horn to agitate the sample, keeping the screen free from debris. Using this method, we have been successful in ridding the pollen sample of debris smaller than 10 microns. This paper presents the results of an investigation of the potential effects of sonication on pollen preservation. We selected 12 fresh pollen types based on a unique range of morphological characteristics, and subjected this assemblage to sonication for different lengths of time and at variable output intensities. Our study demonstrated that when used for 3 minutes at low to medium output intensities, a sonicating disruptor horn can aid in the removal of unwanted debris without considerably damaging pollen grains. We also did not find evidence that pollen colliding with other debris in a sample increased levels of degradation. Analysis on the efficacy of sonication-assisted sieving demonstrated that more tiny debris was removed from the sample at higher frequencies and over increasing durations.


ISSN: 0191-6122
EISSN: 1558-9188
Serial Title: Palynology
Serial Volume: 42
Serial Issue: 4
Title: Efficacy of sonication-assisted sieving on Quaternary pollen samples
Affiliation: Texas A&M University, Department of Anthropology, College Station, TX, United States
Pages: 466-474
Published: 20181002
Text Language: English
Publisher: American Association of Stratigraphic Palynologists, Dallas, TX, United States
References: 12
Accession Number: 2019-014596
Categories: PaleobotanyQuaternary geology
Document Type: Serial
Bibliographic Level: Analytic
Illustration Description: illus. incl. 2 tables
Country of Publication: United States
Secondary Affiliation: GeoRef, Copyright 2020, American Geosciences Institute. Abstract, Copyright, American Association of Stratigraphic Palynologists. Reference includes data from GeoScienceWorld, Alexandria, VA, United States
Update Code: 201910
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