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Crystalline organization of the fibrous prismatic calcitic layer of the Mediterranean mussel Mytilus galloprovincialis

Antonio G. Checa, Carlos M. Pina, Antonio J. Osuna-Mascaro, Alejandro B. Rodriguez-Navarro and Elizabeth M. Harper
Crystalline organization of the fibrous prismatic calcitic layer of the Mediterranean mussel Mytilus galloprovincialis (in Biomineralization and biomimetic materials, Alejandro B. Rodriguez-Navarro (prefacer), Wolfgang W. Schmahl (prefacer) and Manuel Prieto (prefacer))
European Journal of Mineralogy (August 2014) 26 (4): 495-505

Abstract

The outer layer of the shell of members of the genus Mytilus is made of long, slender fibres of calcite (some 1-2 mu m wide and hundreds of mu m long), which reach the internal surface of the shell at an angle. This microstructure has been called anvil-type fibrous calcitic and its organization, crystallography and relationships to the organic phase are poorly known. We have studied the outer calcitic layer of the Mediterranean mussel M. galloprovincialis by means of optical and scanning and transmission electron microscopy (SEM and TEM), X-ray diffraction (XRD) and atomic-force microscopy (AFM). SEM data from other species have also been gathered. All data together imply that the material is extremely well ordered both from the morphological and crystallographic viewpoints. The XRD pole figures show that there are discrete 001 and 104 maxima; therefore, the material has a well-defined sheet texture. In living animals there is an organic membrane (surface membrane) that coats the inner surface of the shell. TEM sections of the decalcified material show that this mainly proteinaceous surface layer is internally laminated and fills all the spaces left between the growing fibres. Every fibre is a monocrystal with three well developed {104} rhombohedral faces at its growth end. One of these faces is directly in contact with, and strictly parallel, to the sublayers of the surface membrane and thus to the inner shell surface. AFM experiments consisting on growing calcite onto shell pieces in which the surface membranes are preserved, show that the calcitic fibres of the shell easily regrow across the membrane, demonstrating that it is permeable to ions. In this way, prisms are able to grow despite the existence of the intermediate membrane in the living animal. Additional experiments of calcite growth onto the inner side of the surface membrane show that crystals grow onto their {104} surfaces. The surface membrane is responsible for the high degree of ordering of the fibrous calcitic layer, because it stabilizes the orientation of a rhombohedral surface, once this is parallel to the protein sublayers. This is one of the very few cases in which the influence of the organic matter on the organization of microstructures can be demonstrated.


ISSN: 0935-1221
EISSN: 1617-4011
Serial Title: European Journal of Mineralogy
Serial Volume: 26
Serial Issue: 4
Title: Crystalline organization of the fibrous prismatic calcitic layer of the Mediterranean mussel Mytilus galloprovincialis
Title: Biomineralization and biomimetic materials
Author(s): Checa, Antonio G.Pina, Carlos M.Osuna-Mascaro, Antonio J.Rodriguez-Navarro, Alejandro B.Harper, Elizabeth M.
Author(s): Rodriguez-Navarro, Alejandro B.prefacer
Author(s): Schmahl, Wolfgang W.prefacer
Author(s): Prieto, Manuelprefacer
Affiliation: Universidad de Granada, Departamento de Estratigrafia y Paleontologia, 18071 Granada, Spain, Granada, Spain
Affiliation: Universidad de Granada, Departamento de Estratigrafia y Paleontologia, Granada, Spain
Pages: 495-505
Published: 201408
Text Language: English
Publisher: Schweizerbart'sche Verlagsbuchhandlung (Naegele u. Obermiller), Stuttgart, Germany
References: 39
Accession Number: 2014-082811
Categories: Mineralogy of non-silicatesInvertebrate paleontology
Document Type: Serial
Bibliographic Level: Analytic
Illustration Description: illus.
Secondary Affiliation: Ludwig-Maximilians-Universitaet Muenchen, DEU, GermanyUniversidad de Oviedo, ESP, SpainUniversidad Complutense de Madrid, ESP, SpainUniversity of Cambridge, GBR, United Kingdom
Country of Publication: Germany
Secondary Affiliation: GeoRef, Copyright 2017, American Geosciences Institute. Abstract, copyright, Schweizerbart'sche Verlagsbuchhandlung. Reference includes data from GeoScienceWorld, Alexandria, VA, United States
Update Code: 201443
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