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Expressed sequence tag profiles from calcifying and non-calcifying cultures of Emiliania huxleyi

Thomas M. Wahlund, Xiaoyu Zhang and Betsy A. Read
Expressed sequence tag profiles from calcifying and non-calcifying cultures of Emiliania huxleyi (in Advances in biology, ecology and taphonomy of extant calcareous nannoplankton, Maria V. Triantaphyllou (prefacer))
Micropaleontology (December 2004) 50, Suppl. 1: 145-155

Abstract

Expressed Sequence Tag (EST) analysis is a powerful means for evaluating gene expression and discovering novel genes. The method is relatively simple and particularly important for species where knowledge of the genome is unavailable or limited. In this study, we compare EST profiles of Emiliania huxleyi cultures grown under conditions that promote biomineralization and coccolithogenesis (F/50 media) and conditions that reduce these processes (F/2 media). A total of 3,527 clones from the F/50 and 4,116 clones from F/2 library were randomly selected and sequenced, resulting in a unigene set of 4,057 unique sequences comprised of 540 contigs and 3517 singletons. More than 40% of the expressed genes showed similarity to known genes from other organisms deposited in GenBank (e>10 (super -2) ) and have been functionally categorized. The comparative data provide cues for further studies pertaining to genes and proteins involved in biomineralization and coccolithogenesis, and taken together serve as a valuable resource for the coccolithophorid research community.


ISSN: 0026-2803
Coden: MCPLAI
Serial Title: Micropaleontology
Serial Volume: 50, Suppl. 1
Title: Expressed sequence tag profiles from calcifying and non-calcifying cultures of Emiliania huxleyi
Title: Advances in biology, ecology and taphonomy of extant calcareous nannoplankton
Author(s): Wahlund, Thomas M.Zhang, XiaoyuRead, Betsy A.
Author(s): Triantaphyllou, Maria V.prefacer
Affiliation: California State University at San Marcos, Department of Biological Sciences, San Marcos, CA, United States
Affiliation: University of Athens, Athens, Greece
Pages: 145-155
Published: 20041201
Text Language: English
Publisher: American Museum of Natural History, New York, NY, United States
Meeting name: International Nannoplankton Association workshop on Extant coccolithophorid research
Meeting location: Heraklion, GRC, Greece
Meeting date: 20031001Oct. 1-6, 2003
References: 50
Accession Number: 2006-007127
Categories: Paleobotany
Document Type: Serial Conference document
Bibliographic Level: Analytic
Illustration Description: illus. incl. 4 tables
Country of Publication: United States
Secondary Affiliation: GeoRef, Copyright 2017, American Geosciences Institute. Abstract, Copyright, Micropaleontology Project. Reference includes data from GeoScienceWorld, Alexandria, VA, United States
Update Code: 200604
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