The Tianshan orogen is divided into the Northern, Central and Southern Tianshan tectonic zones by the northern and southern sutures on both sides of the Central Tianshan Tectonic Zone. The eastern segment of the Central Tianshan Tectonic Zone is characterized by the presence of numerous Precambrian metamorphic blocks and is unconformably overlain by Ordovician–Silurian and late Palaeozoic strata. The Precambrian Kumishi and Pargantag metamorphic blocks are the largest older blocks in the eastern segment of the Central Tianshan Tectonic Zone, consisting mainly of metamorphic granitoids and sedimentary rocks in greenschist to amphibolite facies. There are two major lithological assemblages of the metamorphic granitoids: (1) quartz dioritic gneisses, and (2) granodioritic–monzogranitic gneisses with a minor amount of tonalitic and syenogranitic gneisses in both the Kumishi and Pargantag blocks. The quartz dioritic gneisses are characterized by low Sr/Ce (< 5.3) and Sr/Y (< 28), relatively high Mg no. (51.0–57.0), K2O (2.65–4.04 wt %) contents and εNd(t) values (−2.37–5.84), and negative Nb and Zr–Hf anomalies, as well as relatively flat chondrite-normalized REE patterns with slightly negative Eu anomalies, suggesting that the quartz dioritic gneisses were derived from partial melting of a depleted mantle source enriched by fluids and sedimentary melts from the subducted slab. However, most of granitic gneiss samples display high K2O contents, low Al2O3/(FeO* + MgO + TiO2) values, and relatively flat chondrite-normalized REE patterns with intensively negative Eu anomalies. Integrated low εNd(t) values and older TDM model ages suggest that crustal materials played a significant role in the petrogenesis of these granitoid gneisses and that they were mainly derived from the partial melting of calc-alkaline mafic to intermediate rocks in the crust. Also, variations in geochemical features between the Kumishi–Gangou and Pargantag regions, such as Zr and Hf, may reflect geographic variability in the development of coeval granitic magmas. Tectonic discrimination for granitoid, using trace elements, together with Nd isotopic data, demonstrates that these granitoid gneisses in the eastern segment of the Central Tianshan Tectonic Zone formed in a continental margin arc during late Mesoproterozoic times.