The aim of this study is to describe the biostratigraphic, chronostratigraphic and ecostratigraphic relationships of the Neogene sequence in the Adana Basin. The Adana Basin is located in southern Turkey, and bordered by the Tauride Orogenic Belt to the north, the Amanos Mountains to the east, the Mediterranean coast to the south and the Ecemiş Fault Zone to the west. From base to top, the Neogene sequence consists of the Gildirli Formation (continental redbeds), the shallow marine Kaplankaya Formation, the reefal limestones of the Karaisali Formation, the shales of the Güvenç Formation (slope to deep marine), a thick submarine fan complex (Cingöz Formation), the shallow marine and fluvio-deltaic Kuzgun Formation and the shallow marine lagoonal–continental Handere Formation. The planktonic foraminiferal biozones identified within the Neogene sequence of the Adana Basin are Globigerinoides trilobus and Praeorbulina glomerosa curva (Burdigalian), Globorotalia fohsi peripheroronda/Orbulina suturalis (Langhian), and Globorotalia mayeri (Serravallian). The Late Tortonian is characterized by the first occurrence of Globorotalia suterae. There are no planktonic foraminiferal zones in the Messinian, but this level may be correlated with a non-distinctive zone in the Mediterranean region. The Pliocene is represented by the Sphaeroidinellopsis Acme Zone.