Fossil plants are described from the upper part of the Devonian Lolén Formation, Sierra de la Ventana, Buenos Aires Province, Argentina, in the area of Estancia Las Acacias. The sequence is composed mainly of dark grey shales, and fossils were found in a single horizon where thin inter-layered beds of fine reddish-brown micaceous sandstones appear where the environment of marine deposition became more shallow. The age of the Lolén Formation is presently established on the basis of brachiopods, these being characteristic elements of the Malvinokaffric realm from the Gondwana Lower Devonian (Emsian). The fossil plants are remarkably preserved given that they are in rocks that have undergone intense deformation. The plants are identified as Haplostigma sp. and Haskinsia cf. H. colophylla, and suggest a Middle Devonian age (Givetian) for the fossil-bearing levels. Haskinsia, identified on the basis of leaf morphology, is the first well-delimited Middle Devonian lycopsid genus described from Argentina, and the record from the most southerly palaeolatitude. During the Middle Devonian, Haskinsia was distributed in tropical, warm temperate and high southern latitude, ?cool temperate zones.