Sediment surface death assemblages of recent testate amoebae (Arcellacea) are reported from nine sites in Lake Superior and Lake Michigan. These are the first profundal sediment-water interface samples of testate amoebae described from either of the Great Lakes which provide valuable insight on deep-water, large-lake assemblages. Centropixid strains were present to abundant in shallower, nearshore sites (up to 66 m water depth). Assemblages at depths >40 m were dominated by Difflugia oblonga “tenuis.” The shallowest sample (26 m) was dominated by Centropyxis aculeata “discoides” and Difflugia oblonga “tenuis.” Over 100 tests per sample were observed from >100 m. Density of tests appears to be constrained by lithology rather than water depth. The deepest site (325 m) yielded low foraminiferal abundances. This pilot study provides a first step towards documenting the distribution of testate amoebae in the Great Lakes.