The significance of fossil testate amoebae having organic shells and their application for biostratigraphical and paleoecological studies are unexplored fields, although these organisms are extensively used as bioindicators in ecological studies of Recent and Holocene environments. Some taxa of fossil testate amoebae with organic (chitinous, pseudochitinous, or proteinaceous) shells were recovered in Turkey from a series of palynological samples, ranging in age from Late Cretaceous to Holocene. A total of 202 samples were processed from sediments from 11 sites representing fluvial flood-plain, lacustrine, estuarine, and peat-swamp as well as shallow and deep marine facies. Nineteen taxa of the genera Arcella, Centropyxis, Frenzelina, Microcometes, Nebela, Phryganella, Pyxidicula, and Sphenoderia were recorded from the Late Cretaceous, Lower−Middle Eocene, Lower Miocene, and Quaternary sediments. The literature on fossil organic-walled testate amoebae records was also reviewed and compared with the findings in this study.