Two species, Spirobolivina papillosa n. sp. and Spirobolivina retorta n. sp., are described from the Bizerte Lagoon (Tunisia), a Mediterranean transitional ecosystem. Two additional species, S. curta (Cushman, 1933) and S. minutaMcCulloch, 1977, are also recorded from this lagoon. The identification and documentation of the new species are followed by a comparison with all the species belonging to the genus Spirobolivina and with selected species assigned to the genera Bolivinopsis and Spiroplectoides. Spirobolivina spp. are uncommon in Mediterranean modern environments and their ecology and paleoecology are poorly understood.