In this study, a clinoptilolite modified with apolaccase was used to adsorb Reactive Black 5 (RB5) dye from aqueous solution using the batch procedure. The influences of pH, contact time, temperature and absorbent dosage on the adsorption were investigated. The optimum adsorption was obtained at pH = 6, contact time = 60 min, temperature = 25°C and adsorbent dosages of 1.62 and 1.59 mg/50 mL per gram of clinoptilolite and of apolaccase-modified clinoptilolite (LMC), respectively). The adsorption experimental data fitted both the Langmuir and Freundlich isotherm models well. In addition, pseudo-first-order and pseudo-second-order kinetics were used to study the kinetics of RB5 dye adsorption onto natural clinoptilolite and LMC. Adsorption appears to follow pseudo-second-order kinetics with a high correlation coefficient. Thermodynamic parameters such as changes in the free energy (ΔG°), enthalpy (ΔH°) and entropy (ΔS°) of adsorption were calculated. The thermodynamic parameters indicate that the adsorption of RB5 dye onto LMC was less spontaneous, feasible and endothermic. The LMC can be used as an alternative low-cost adsorbent for the dye removal from aqueous solutions.