The removal of enrofloxacin, a fluoroquinolone antibiotic, from aqueous solution by adsorption onto bentonite was investigated in this study. The effects of initial concentrations, contact time and temperature on the adsorption of enrofloxacin were studied via batch experiments. The adsorption equilibrium was achieved within 60 min for all studied concentrations. The adsorption capacity increased with the increase of initial concentration within a concentration range. Higher temperatures were favourable for the adsorption. The change of Gibbs free energy (ΔGo), change of enthalpy (ΔHo) and change of entropy (ΔSo) were evaluated and the results indicate that the adsorption should be an endothermic and spontaneous process. The Langmuir isotherm model fitted to the experimental data better than the Freundlich model. The adsorption follows the pseudo-second order kinetic model.