The Natiscotec outcrop on Anticosti Island, Canada (Ellis Bay Formation, Laframboise Member, Hirnantian, Late Ordovician), exposes a patch reef some 20–30 m in diameter, 2–3 m thick, with abundant rugose corals, as well as favositids and heliolitids. Reef capping and flanking sediments include typical Hirnantian brachiopods, such as Hirnantia, Hindella, and Eospirigerina. Within the reef peloidal microbialites encrusting bryozoan colonies are common. The bryozoan fauna includes three cystoporates, seven trepostomes, and three phylloporines. Two genera and two species are new: the cystoporate Natiscotecella tenuis n. gen. and n. sp. and the phylloporine Dilaminocladia natiscotecensis n. gen. and n. sp. Three more species are also new: the cystoporates Ceramopora clara n. sp. and Acanthoceramoporella spinigera n. sp. and the trepostome Revalotrypa honguedensis n. sp. Furthermore, we identify the three trepostomes Atactoporella aff. ortoni (Nicholson, 1874), Hallopora elegantula (Hall, 1852), and Monotrypella cf. aequalis Ulrich, 1882, and a phylloporine Parachasmatopora porkunensis Lavrentjeva, 1985. Four species are identified at generic level and in open nomenclature: the three trepostomes Lioclemella sp., Calloporella sp., and Trepostomata sp. and a phylloporine ? Ralfinella sp. The bryozoan fauna shows some affinities with the Late Ordovician fauna of Scandinavia. Stable carbon isotope investigations from brachiopod shells of the same outcrop yield values for δ13C of up to +6.7‰, which represent the highest values reported from the Anticosti succession so far. This indicates that the stratigraphic position of the outcrop is at or close to the peak of the globally recognized Hirnantian δ13C excursion.