Vertical incidence seismic data were collected along a 300 km-long profile across the northwestern flank of the Trans-Hudson Orogen in Saskatchewan (line S2b). The present study integrates the seismic data with previously published geological maps and recent results from the La Ronge – Lynn Lake Bridge Project that provide new constraints on the lithological, structural, and tectonic framework of this collisional zone. An interpretative section, based on the seismic reflectivity and surface geological constraints, is presented. The integrated data suggest the following key elements. (1) Although an early foreland-vergent accretion history is suggested by surficial geological constraints, the predominant fabric is reflected by penetrative hinterlandward vergence. (2) A zone of more reflective lower crust with hinterlandverging fabric may represent “fossil” reflectors from a pre-collisional accretionary phase. (3) The Archean to Paleoproterozoic Sask Craton may extend in the lower middle crust to at least beneath Rottenstone Domain, consistent with what is observed along strike on other Lithoprobe seismic profiles. (4) The Needle Falls and Parker Lake shear zones do not appear to be associated with obvious reflectors, consistent with observations from Reindeer Lake, suggesting that they merely rework the Wathaman Batholith intrusive margin and are not fundamental sutures. (5) The La Ronge and Rottenstone domains show nearly identical seismic properties, consistent with recent mapping results in the Reindeer Lake area that suggest that they are temporally and, at least in part, lithologically related in the area covered by, and to the east of, seismic line S2b.