Nd and Pb isotopes from the Lake of the Woods greenstone belt indicate the presence of three distinct reservoir sources: old enriched crust (>3.0 Ga); pre-2.7 Ga, homogeneous depleted mantle; and post-2.70 Ga heterogeneous mantle. εNd values of +1.1 to +2.3 for ultramafic to felsic metavolcanic rocks (2.74–2.72 Ga) indicate derivation from depleted mantle. The εNd value of –0.9 for younger turbidite (2.71 Ga), in conjunction with detrital zircon ages ranging from 2.72 to 3.0 Ga, indicates detritus from local greenstone belt sources (depleted mantle) mixed with an older crustal source. Post-2.70 Ga heterogeneity is demonstrated by εNd values ranging from –0.4 to +0.4 in shoshonitic to calc-alkaline metavolcanic rocks and +2.1 in a coeval ultrapotassic pluton. Pb isotopes from the pluton indicate derivation from a depleted mantle reservoir with an initial 207Pb/204Pb of 14.52, an initial 206Pb/204Pb of 13.29, and μ1 of 7.86. Isotopic comparison with post-2.70 Ga potassic suites from across the Superior Province indicates widespread mixing between depleted mantle and enriched end members. The enriched end member has isotopic characteristics of rocks derived from old crustal terrains, such as the Winnipeg River and Opatica subprovinces. This type of isotopic heterogeneity could be the result of crustal contamination or derivation from metasomatized mantle. Contamination of the mantle wedge by influx of fluids derived from partial melting of isotopically evolved, subducted sediments is favoured for the Superior Province potassic suite, because elevated concentration of Sr, Nd, and Pb in conjunction with primitive Mg#s suggest only limited crustal contamination has occurred.