Metamorphosed and ductilely deformed sedimentary, plutonic, and volcanic rocks of the Nisutlin and Anvil assemblages make up the Yukon–Tanana terrane in the Teslin tectonic zone study area. The Nisutlin assemblage consists of siliceous schist–quartzite and graphitic phyllite that share a primary depositional contact, and Early Mississippian tonalite to quartz diorite that intrudes the siliceous schist–quartzite and possibly the graphitic phyllite. The Anvil assemblage includes metagabbro and mafic schist–greenstone that share an intrusive contact relationship. Tonalite to quartz diorite of the Nisutlin assemblage is characterized by minor zircon inheritance with an average Proterozoic age, εNd(350 Ma) values of −2.5 to −6.2, and Nd model ages of 1.50–1.79 Ga. These data suggest that the magmatic bodies have inherited a component of continentally derived material. Primary contact relationships and age data indicate that the Nisutlin assemblage had formed by Mississippian time, and regional correlations show that this assemblage makes up a large part of the Yukon–Tanana terrane of southern Yukon. Assembly of the Nisutlin assemblage by Mississippian time indicates that it did not form as a late Paleozoic and early Mesozoic subduction melange, and it suggests that its tectonic fabrics did not result from the progressive growth of a Permo-Triassic subduction complex. We suggest that the Nisutlin assemblage was part of a crustal block that lay outboard of North America in Mississippian time, and that it lay in the hanging-wall plate of a Permo-Triassic subduction zone as a relatively coherent assemblage, rather than forming within the zone as a subduction complex.