A zone of granulites, defined by an orthopyroxene-in isograd and extending more than 100 km in length and about 10 km in width, occurs near the southern margin of the Quetico Subprovince, north of Manitouwadge, Ontario. Mineral assemblages in metasedimentary rocks and associated migmatites consist of quartz, plagioclase, garnet, orthopyroxene, biotite, cordierite, sillimanite, K-feldspar, hercynite, magnetite, ilmenite, and other accessory phases. Minor mafic gneisses and calc-silicate pods or lenses are also present. From equilibrium phase relations and thermobarometry, the granulites experienced a thermal-peak event (4–6 kbar (1 bar = 100 kPa), 680–770 °C, a(H2O) of 0.15–0.25 and fO2 of 1–2 log units above the FMQ buffer) in association with D2 deformation, followed by a retrogression (550–660 °C and 3–4 kbar) and a later hydrothermal alteration (1–2 kbar and 200–400 °C). The distribution and calculated peak metamorphic conditions of the granulite zone in the Quetico Subprovince are similar to those of granulites in the English River Subprovince and other proposed accretionary terranes. The low-pressure, high-temperature metamorphism in the Quetico Subprovince is interpreted to be related to both crustal thickening and addition of heat from subduction-related magmatism.