Ordovician conodont faunas from the fine-grained clastic Road River Group in northern Yukon Territory contain a mixture of species from both warm- and cold-water regions. This group in southwestern Northwest Territories also has mixed faunas, whereas conodonts from the carbonate facies are more characteristic of the warm-water regions.Six conodont associations and biozones are identified from the Yukon. Some, such as the early Llanvirn "Cordylodus" horridus – Spinodus spinatus association, contain reworked elements. The Pygodus serra Zone (middle–late Llanvirn) is within the upper P. tentaculatus Zone and well below the G. euglyphus Zone. Genera include Ansella, Periodon, Protopanderodus, Pygodus, and Walliserodus.At Peel River, conodonts are within the P. pacificus Zone. The Blackstone River conodonts occur above the P. pacificus Zone and below the G. persculptus Zone (?) and include Oulodus rohneri, Plectodina florida, and Noixodontus. Amorphognathus ordovicicus, Gamachignathus ensifer, O. ulrichi, and Plectodina tenuis occur in both faunas. These represent Fauna 12, found in late Richmondian strata, rather than the Gamachian Fauna 13 and are assigned to the G. ensifer Zone; both occurrences of G. ensifer are biohorizons. The C.? extraordinarius and G. persculptus zones are not recognized at Peel and Rock rivers and Tetlit Creek.The Ozarkodina n. sp. A – Icriodella sp. B association occurs at Pat Lake between the G. persculptus Zone (?) and the underlying P. pacificus Zone. The Ozarkodina have a Silurian aspect but must be regarded as Ordovician.The Ordovician–Silurian boundary in the Road River Group of the Yukon is best defined using graptolites because of the rare occurrence of conodonts.