Since its discovery in 2008, the opal deposit in the Wegel Tena area of Ethiopia, located within an Oligocene ignimbritic trap, has become a major provider of precious opal. This study provides new field evidence and laboratory results that reinforce the pedogenetic model for gem opal formation. In the eight mines investigated, opal occurs in lenticular horizons within an unwelded ignimbrite bed. Moreover, several deposits are superimposed in the sequence, indicating that gem opal formation occurred several times in the same place. We observed that, in contrast to the other beds, the opal-bearing horizons are strongly weathered: volcanic glass is altered to smectite, rocks with a granular microstructure are apparent, and cavities are covered by illuvial clays. These features are typical of pedogenetic processes. Plant fossils in the opals reveal a well preserved cellular structure composed of chalcedony. These observations are consistent over all the mines, supporting the pedogenetic model for precious opal formation in the Wegel Tena area.