Multiple orders of depositional cyclicity in the Mayes Group of northeastern Oklahoma are delineated by refined depositional facies associations and stratigraphic surfaces. Facies associations include deep subtidal facies, shallow subtidal facies (including distal and proximal subfacies), carbonate shoal facies, and shoal crest facies. The Mayes Group records a primary transgressive–regressive depositional cycle bounded below by a major unconformity (sub-Mayes unconformity) and above by an important provincial conodont biostratigraphic boundary and widespread flooding surface at the base of the Fayetteville Shale. Within the Mayes Group, two secondary transgressive–regressive depositional cycles are separated by an interpreted unconformity. The lower Mayes cycle comprises the Bayou Manard and Lindsey Bridge members of the Pryor Creek Formation, whereas the Ordnance Plant Member is grouped with the Hindsville Formation in the upper Mayes cycle. Present in both the lower and upper Mayes cycles are high-frequency shallowing-upward cycles bounded by flooding surfaces. Evaluating the distribution of facies and stratigraphic surfaces within a framework of multiple orders of depositional cyclicity is essential to interpreting the geologic evolution of the southern mid-continent during the Meramecian and Chesterian, and impacts oil and gas production by improving our understanding of reservoir compartmentalization.