Preliminary results of the MIDTAL project: a microarray chip to monitor toxic microalgae in the Orkney Islands, UK
Published:January 01, 2013
J. D. Taylor, M. Berzano, J. Lewis, L. Percy, L. K. Medlin, 2013. "Preliminary results of the MIDTAL project: a microarray chip to monitor toxic microalgae in the Orkney Islands, UK", Biological and Geological Perspectives of Dinoflagellates, J. M. Lewis, F. Marret, L. R. Bradley
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Harmful algae can cause economic damage to ﬁsheries and tourism. Additionally, toxins produced by harmful algae and ingested via contaminated shellﬁsh can be potentially fatal to humans. Monitoring these harmful algae can be difﬁcult as determining cell morphology by light microscopy may be insufﬁcient to give deﬁnitive species attribution. The goal of the EU FP7 project MIDTAL (microarrays for the detection of toxic algae) was to achieve rapid species identiﬁcation using species-speciﬁc probes for rRNA genes in a microarray chip format. Field samples from the Orkney Islands, an area of the UK that has a number of nuisance and toxic species, were tested with the second generation of the microarray chip. Species-speciﬁc probes were looked at for the toxin-producing dinoﬂagellates Alexandrium tamarense Group III (North American clade) and Dinophysis acuta and also general class probes for Dinophyta, Heterokontaphyta and Prymnesiophyta over the course of a year. These were compared with light microscopy cell counts. A good agreement in determining presence and absence between the methods was found. The second generation microarray is potentially more sensitive than cell counts. However, further work is needed to ensure that the microarray signal for each species provides an accurate quantitative assessment.
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Biological and Geological Perspectives of Dinoflagellates
This volume provides an overview of current research on fossil and modern dinoflagellates, as well as highlighting research areas for future collaboration, following the DINO9 International Conference in Liverpool. The volume is organized into four themes, with a review paper for each theme written by the key-note speaker. Each theme also includes a future research foci note following discussion during the conference. The contributions are organized into the following sections: environmental change, ecology/palaeoecology, life cycles and diversity, and stratigraphy and evolution. Also included are notes from two workshops: culture experiments and dinocysts as palaeoceanographic tracers. This volume will be of interest to both the biological and Micropalaeontological communities.